Particle sizing

SEC-MALS - Dawn Heleos, rEX (Wyatt Technology) & UV absorbance detector (Shimadzu)

Multi-angle light scattering (MALS) determination in-line with a chromatographic setup allows to monitor the absolute molecular weight of proteins and complexes as they elute from the column. The light scattering detector in our setup is the Wyatt Dawn Heleos and concentration is measured using a refractive index detector (Wyatt rEX) or a UV absorbance detector (Shimadzu). The chromatography is done by a Shimadzu HPLC.